The PYROGENT™–5000 assay is a quantitative, kinetic assay for the detection of Gram-negative bacterial endotoxin. A sample is mixed with the reconstituted LAL reagent, placed in the incubating absorbance plate reader, and automatically monitored over time (via WinKQCL™ Software) for the appearance of turbidity.
The PYROGENT™–5000 assay is perfect for laboratories needing to process large numbers of samples. This assay is performed in a 96-well plate allowing many samples to be processed at one time. It is ideal for water samples, large volume parenterals and water rinse from medical devices. The PYROGENT™–5000 assay is measured at the 340 nm wavelength and has a sensitivity range from 0.01 to 100.0 EU/ml. An incubating plate reader is required to run this assay.