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ProteinSEQ^™ Protein Quantitation System: Bringing the Sensitivity of qPCR to Protein Quantitation



Wesley Straub, PhD, Sr. Pharmaceutical Analytics Product Manager, Thermo Fisher Scientific

Wesley Straub is a Senior Pharma Analytics Product Manager at Thermo Fisher Scientific and is responsible for development of new platforms for protein analytics. Wesley has more than 15 years of protein chemistry and characterization experience. Prior to joining Life Technologies,now part of Thermo Fisher Scientific, Wesley managed software and consumables for the label-free protein platform at ForteBio until its acquisition by Pall Corporation. He also led downstream development efforts for the purification and characterization of novel IgG and non-antibody-based biotherapeutics and biosimilars. Wesley received a Bachelor's of Science in Chemistry from Boston University, and a PhD in Pharmaceutical Chemistry from UCSF. As a post-doctoral scholar at the University of Frankfurt, Wesley developed novel forms of biosynthetic protein labeling, and described new paradigms for the structure-function relationship of stem cell transcription factors.

Webinar Info

The removal of host cell impurities is a critical step in the production of biopharmaceuticals. Traditional methods of quantifying residual host cell DNA, Protein A and Host Cell Protein impurities have been limited by narrow dynamic range, lack of sensitivity and specificity, and slow time -to-results. Life Technologies has responded to these challenges with a revolutionary new solution. The ProteinSEQ^™ Protein Quantitation System is a breakthrough immunoPCR technology for sensitive and reproducible quantitation of Protein A or host cell impurities. In this session, learn about the key features and benefits including:

• Automated analyte extraction for most efficient workflow, higher run-to-run reproducibility, and minimized assay failure rate
• Broad dynamic range of 4 logs
• High sensitivity with an LOQ of 0.2 ng/mL for generic CHP HCP and 0.512 pg/mL for Protein A
• Consistent performance across diverse sample types
• Same-day results, typically in less than 5 hours, and less than ten minutes of hands-on time, after sample prep

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• Area of Bioprocessing *
  Upstream (i.e. cell line development, cell culture, cell therapy)Downstream (i.e. purification, chromatography, pre-packed process scale columns)

  Pharma Microbiology (i.e. QC micro)Biopharma Process Analytics (i.e. bacterial/fungal id, mycoplasma detection, micro virus detection)
• The contaminants you currently test for are (check all that apply): *
  MycoplasmaMouse minute virusVesivirus

  Other virusesNot presently testing for contaminants, but will have a need in the near future
• If you checked "Other Viruses" above, please list all:
• Which of the following impurities do you currently analyze? *
  Residual host cell DNA testingResidual Protein AHost Cell Proteins
• If you checked "Residual host cell DNA testing" above, please check all that apply
  CHOE.coliMDCKHuman

  PicchiaVeroOther
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