Be an LC-MS Whiz: How to Optimize Sample Preparation, Select Mobile Phases and Columns for Enhanced Separation
Vivek Joshi, Ph.D.
Tuesday, June 16, 2015
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Chapters
Introduction
Agenda
LC-MS is used across a wide variety of Markets
Success in LC-MS is all about the Analyte
Three key components of success in LC-MS
Key things impacting LC-MS performance
Sample Preparation
Importance of sample preparation
Trends in sample preparation
Particle retention by various syringe filters
Pore size versus membrane morphology
Drug recovery after Protein Precipitation
SPE troubleshooting: Common problems and some solutions
MS extractables from syringe filters
LC-MS extractables from syringe filters
Any surface in contact with sample can add contaminants to the sample
Mobile Phase Components
Importance of Mobile phase quality for LC-MS analysis
General considerations
For LC-MS, use LC-MS grade solvents
All LC-MS grade solvents are not created equal
Even MS grade waters are different from each other
Storage conditions impact quality of ultrapure water
Buffer salts impact LC-MS analysis
Particles in mobile phase = High backpressure
Chromatography Column
Appropriate column selectivity is needed for sufficient resolution
Most commonly used HPLC column chemistries
Why do we choose a certain HPLC column?
How to improve sensitivity in LC-MS analysis?
Column robustness and lifetime
Column bleeding causes noisy background
Sample preparation and analysis time
Minimum sample prep, good chromatography
Summary
Acknowledgements
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