Shimadzu’s Nexera-e comprehensive 2D-HPLC provides a novel separation method for complicated sample matrices, including pharmaceutical impurities, lipids, peptides, and natural products. By combining two independent separation modes orthogonally together with a dual loop/dual valve alternate switching design, the Nexera-e 2D-HPLC provides separation of the mixture no matter how complex. Easy-to-use ChromSquare data analysis software provides all information for data analysis including contour plotting, MS spectra and second dimension chromatogram in one easy-to-view window.