Validation of the Performance of the PerkinElmer FL 6500 and FL 8500 Fluorescence Spectrometers

For certain analyses, fluorescence is the method of choice due to its superior sensitivity compared to UV/Visible absorption spectrophotometry.  Fluorescence has the potential to be more sensitive- provide that the material under investigation has high quantum efficiency at the wavelengths being used.  Fluorescence is a relative technique- this means that a quantitative measurement requires a comparative measurement against a material of known concentration.  Validation of the performance of this technique is more challenging compared to other, more absolute, techniques such as absorption spectroscopy. 

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