ProBioGen announced that the Japan Patent Office (JPO) issued the first patent for the company’s DirectedLuck® technology. The powerful gene delivery system is based on a highly active transposase fusion variant that enables epigenetic targeting and a carefully designed transposon.
This technology provides bulk pools that not only achieve titers of up to 13 g/L for different antibody formats but also exhibit exceptional expression stability over more than 50 population doublings. This makes these pools well-suited for large-scale manufacturing.
DirectedLuck® has been successfully applied at ProBioGen to generate hundreds of producer cell lines for clients and is endorsed by ProBioGen’s licensees. In addition, DirectedLuck® can be used to create stable packaging cell lines for viral vectors and in gene and cell therapy.
About DirectedLuck®
DirectedLuck® builds on a transposase's principle but with additional major improvements. It has been optimized to recognize specific chromatin marks, guiding the transgene to genomic regions with highest transcriptional activity, which ensures exceptionally high protein expression and maximum stability. The clone pools generated within days to weeks are highly representative of later clones. This reduces time and hands-on lab work for selecting superior clones for best titers, proven stability and product quality. The DirectedLuck® Transposase is compatible with additional genetic elements in standard expression vector design and can be used with any host cell line from different species and tissue origin. DirectedLuck® has been applied to develop cell lines for standard mAbs, complex glycoproteins and bispecifics and is also applicable beyond protein and virus production in cell and gene therapy.
DirectedLuck® is available for out-licensing. Furthermore, ProBioGen applies DirectedLuck® as a standard tool in clients' service projects at no extra charge.
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