Chromatography is a technique to
separate a mixture. It utilizes phase equilibrium partitioning to isolate
proteins, nucleic acids, and other small molecules in complex mixtures by their varied interactions with stationary and mobile phases. The two main types of chromatography are
Liquid Chromatography (LC) and
Gas Chromatography (GC).
Chromatography may be
preparative or analytical. Pharmaceutical companies use chromatography to prepare large amounts of pure materials and analyze purified compounds for contaminants.
High-performance liquid chromatography (HPLC) can be distinguished from traditional liquid chromatography by its significantly higher operational pressures. Ordinary liquid chromatography often uses gravity to pass the mobile phase through the column. HPLC thus has superior resolving power when separating mixtures. HPLC is commonly used in
protein separation, insulin and enzyme purification and plasma fractionation..
Pharmaceutical Chromatography Supplies
- Cartridges, Syringes and Vials
- Fraction Collectors
- Tips, Fibers and Cleaning Solutions
- Precipitation Plates
- Colum Protection Fluids and Resin
Pharmaceutical HPLC Equipment
- Columns
- Detectors
- HPLC Systems / HPLC Instruments
Chromatography equipment includes all the components needed for separation:
columns, frits, flow cells, pumps, detector, collectors and software to complete systems. Consider the platform being used, its compatibility with other components and the space available in the laboratory when selecting proper equipment.
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