Chromatography Fraction Collector

The fraction collector is typically a rotating rack that can be filled with test tubes or similar containers. It allows samples to be collected in fixed volumes, or can be controlled to direct specific fractions detected as peaks of protein concentration, into separate containers.

Many systems include various optional components. A filter may be added between the mixer and column to minimize clogging. In large FPLC columns the sample may be loaded into the column directly using a small peristaltic pump rather than an injection loop. When the buffer contains dissolved gas, bubbles may form as pressure drops where the buffer exits the column; these bubbles create artifacts if they pass through the flow cells. This may be prevented by degassing the buffers, e.g. with a degasser, or by adding a flow restrictor downstream of the flow cells to maintain a pressure of 1-5 bar in the eluant line.