The Incucyte® CX3 Live-Cell Analysis System is a next-generation imaging instrument designed to enable researchers to study both 2D and 3D cell models dynamically and non-invasively. The CX3 delivers continuous, long-term imaging within a controlled, physiologically relevant environment, empowering scientists to capture real-time insights into cell behavior without disrupting cultures. At the core of its innovation is advanced confocal imaging capability, ensuring clear, detailed images of complex models such as spheroids and organoids. By combining versatility with ease of use, the CX3 enables researchers to address a wide range of biological questions with accuracy and reproducibility.
Features and Benefits
The Incucyte® CX3 incorporates cutting-edge imaging technologies, offering researchers a choice between spinning disk confocal, wide-field fluorescence, high definition phase, and bright-field imaging. Its multiplexing capabilities support up to three fluorescence channels— green, orange, and near-infrared—enabling simultaneous monitoring of multiple parameters in a single experiment. Equipped with an automated turret containing 4x, 10x, and 20x objectives, the system allows seamless switching between magnifications for flexible study of cellular morphology and function.
The multi-user design of the CX3 system features three interchangeable vessel trays, supporting up to six microplates in parallel, making it highly efficient for collaborative laboratory settings. Importantly, the system offers continuous, label-free as well as flfluorescence-basedssays assays, preserving the integrity of living samples while delivering comprehensive data on cell health, proliferation, movement, and phenotypic changes. By automating workflows and maintaining cultures in their physiological state during extended imaging, the CX3 reduces experimental variability, accelerates data collection, and increases research productivity.
Applications
The Incucyte® CX3 Live-Cell Analysis System has wide applicability across a spectrum of cell-based research areas. It is particularly valuable in assays aimed at monitoring cell health and proliferation, enabling researchers to track population dynamics over time. The system also excels in functional studies, capturing details of cell movement, morphology, and interaction in both 2D monolayers and complex 3D culture systems. With its advanced confocal imaging, the CX3 is especially well-suited for studying spheroids, organoids, and other physiologically relevant 3D models, providing critical insights into tumor biology, stem cell differentiation, and tissue development. In addition, it supports diverse workflows for long-term monitoring of experimental conditions, streamlining processes in both academic and translational research laboratories.
By delivering high-quality images and comprehensive data without disturbing living cell systems, the CX3 empowers scientists to make more confident, timely decisions in cell biology, drug discovery, and advanced model development.