Live-Cell Analysis for Quantification of Organoid Growth Dynamics Using Sartorius RUO Growth Factors and Cytokines

[Article Title]

This application note investigates the efficacy of recombinant growth factors in enhancing organoid expansion and allowing researchers meticulous control over culture conditions. Through the cultivation of mouse pancreatic and hepatic organoids in media enriched with Sartorius Research Use Only (RUO) Growth Factors and Cytokines, in combination with the Incucyte® Live-Cell Analysis System, we demonstrate their capacity to significantly enhance and sustain organoid growth for extended periods, outperforming other commercially available alternatives. Furthermore, these RUO reagents enable precise manipulation of concentrations, facilitating the identification of optimal culture conditions and the dissection of individual growth factor functions. This is exemplified through a concentration response curve applied to R-spondin1, showcasing their versatility in advancing organoid research. Download now to learn more.

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