Fast and Efficient Isolation of Small-sized Extracellular Vesicles from Stem Cells Using a Combination of Single-Use Bioreactors, High-Speed- and Ultracentrifugation

[Article Title]

Cells release diverse types of membrane vesicles in the extracellular environment, called extracellular vesicles (EVs), comprising exosomes and microvesicles. Exosomes (30–150 nm) are an important mode of cell-to-cell communication by transferring biomolecules between cells. They can also be used as biomarkers and are investigated as drug delivery vehicles.

Here, we describe a fast and easy isolation process of small EVs from adipose-derived stem cells using high-speed and ultracentrifugation. Cells were cultured in BioBLU® 0.3c Single-Use Bioreactors and controlled by the DASbox® Mini Bioreactor System, allowing high EV production. Clearance of the culture medium was done using the Centrifuge CR22N with Rotor R15A. Further concentration was achieved with the Ultracentrifuge CP100NX and Rotor P32ST. A sucrose cushion enabled rapid isolation of pure and homogeneous small EVs. High-volume rotors reduced repetitive steps, cutting isolation time to under 4 hours—faster than precipitation or filtration.

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