Monitoring monoclonal antibody (mAb) titer, post-translational modifications (PTMs), charge variants, and aggregates often requires multi-dimensional separations to resolve overlapping peaks and identify unknowns. Two-dimensional liquid chromatography, or 2D-LC, combines two complementary separations to resolve complex samples more effectively than a single separation. Switchable, heart-cut, 2D-LC workflows let scientists automate sample collection, clean-up, and desalting, enrich samples to improve signal quality, and toggle between UV for speed and MS for depth.
In this guide from Thermo Fisher Scientific, learn more about how 2D-LC instruments with switchable fluidics support fast and thorough monitoring of mAb critical quality attributes (CQAs) directly from the bioreactor during upstream and downstream processing.