Description
Tandem-repeated ubiquitin-binding entities (TUBEs) were designed using four tandem ubiquitin-associated (UBA) domains from either ubiquilin 1 (UBQLN1) or UV excision repair protein RAD23 homolog A (RAD23A). TUBEs were conjugated to Sepharose for efficient purification of ubiquitylated proteins from cell extracts.