Description
The InTraSeq™ methodology enables researchers to measure proteins (cytoplasmic, surface, and nuclear), including post-translational modifications (PTMs), along with RNA in their single-cell assays. While single-cell RNA sequencing (scRNAseq) has transformed the field of cellular and molecular biology, it only measures RNA transcripts within single cells. This presents a significant challenge, as RNA levels don't necessarily translate to protein abundance or activity. The InTraSeq technology addresses this limitation and can unravel missing information in scRNAseq experiments, uncovering new cell states and crucial biological data in single-cell analyses, especially since RNA does not reflect PTMs. InTraSeq™ F' Conjugate Antibody Cocktail 1 is a mixture of 100 titrated InTraSeq™ F' Conjugates that are conjugated to 10x Genomics Feature Barcodes. This cocktail is only compatible with the 10x Genomics immunostaining protocol Cell Surface & Intracellular Protein Labeling for GEM-X Flex Gene Expression (CG000781). In the 'Intracellular Protein Labeling' section, refer to the 'For Non-lyophilized Antibody' subsection and add 15 µL InTraSeq™ F' Conjugate Antibody Cocktail 1 when preparing the 'Intracellular Antibody Mix'. This cocktail is validated by CST using 10x Genomics GEM-X Flex v1 and GEM-X/Apex Flex v2 (CG000788, CG000789, CG000841, and CG000835).