Description
The Human B Cell Signaling Flow Cytometry Panel can be used to interrogate activation of human B cells among peripheral blood mononuclear cells (PBMCs). CD45 is a pan leukocyte marker. B cells are identified by expression of CD19. Engagement of the B cell receptor (BCR) triggers the tyrosine phosphorylation of CD79A at Tyr182, which is mediated by Src family kinases. Following engagement of the BCR, Syk is recruited to phosphorylated ITAM motifs within the BCR signaling complex, which facilitates Syk activation through phosphorylation of Tyr525/526 within the kinase activation loop.