Poster: Efficient Monoclonal Antibody Aggregate Removal by Hydrophobic Interaction Chromatography (HIC)

Advances in bio-therapeutics are generating more diversified classes of biomolecules that are presenting unique and often difficult purification challenges. To meet industry demand for more differentiated purification tools, a series of HIC resins were developed using extensive user input. Design goals focused on introducing resins addressing typical pain points for current HIC separations such as resolution, capacity and poor product recovery.


The case study presented, shows the optimization of Thermo Scientific™ POROS™ HIC resin use in high throughput screening and subsequently upscaling in both bind-elute and flowthrough mode. The goal of this study was to design a more efficient, robust and cost-effective polish step utilizing HIC as an alternative to the mixed-mode step in the original purification process of a clinical Mab containing >10% aggregates. Here we present the superior performance of our HIC resins in a typical monoclonal antibody aggregate removal process.

Download Document

    Please fill out the information below to request information on the products you selected. If you are already registered, or have requested a quote before, and this information will be filled out for you.

  • Please enter the name of the company or institution you work at.

  • Please enter your mailing address.

  • Please enter the e-mail address you would like to be contacted at.

  • Please enter the phone number you would like to be contacted at.

  • Thermo Fisher is here to help you succeed in taking your discovery to commercial realization. Would you like to have a technical expert contact you to learn more about our Hydrophobic Interaction Chromatography solutions?*

    I would like to receive information about events, products, services, and promotions from Thermo Fisher Scientific and its affiliates: I agree for the Thermo Fisher Scientific group thermofisher.com/legalentities to contact me by email and telephone to inform me of events, products, services, and promotions offered in connection with the brands Thermo Scientific™, Applied Biosystems™, Invitrogen™, Gibco™, Ion Torrent™, Unity Lab Services™, Fisher Scientific™. I can withdraw my consent and unsubscribe at any time by emailing reply@thermofisher.com By submitting my data, I give consent to the collection, processing and use of my personal data in accordance with the Thermo Fisher Scientific Privacy Policy.

  • By submitting this form, you consent to American Pharmaceutical Review storing your email address and contact information, transmitting your contact information to selected trusted partners, and customizing your experience based on the data you enter.